Carboxylated Plates are ideal for promoting the covalent immobilization of compounds with reactive free amino groups. The surface features a negatively charged carboxylated layer, enabling the binding of peptides and other molecules through specific covalent bonds using EDC-mediated amination. This method overcomes several limitations, including the immobilization of small molecules (1,000–5,000 Da), such as peptides, drugs, toxins, or hormones, which may otherwise bind weakly or not at all through physical adsorption. Additionally, it ensures oriented immobilization, maintaining the integrity and accessibility of specific sites, and offers increased storage stability by minimizing the risk of spontaneous desorption.
Carboxyl Treated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.
- Enhanced binding efficiency for small molecules like peptides (1,000–5,000 Da), drugs, toxins, and hormones, which typically struggle with physical adsorption.
- Oriented immobilization, ensuring that molecules retain their structural integrity and maintain accessible active sites for optimal functionality.
- Improved storage stability, significantly reducing the risk of spontaneous desorption, leading to more reliable and long-lasting immobilization compared to physical adsorption methods.
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
- Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 75 to 200 μl
- Packaging: 5 plates in a transparent bag.
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
- Plates Design:
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
- ISO 9001 Certified: Produced under strict quality protocols.
- Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
- SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.
Biomat Carboxylated Plates are offered in three formats to accommodate diverse laboratory needs:
- Carboxylated Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.
- Carboxylated Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.
- Carboxylated Plates 96-Well Solid Plates: Solid format designed for high-performance assays.
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Other
For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.
FOR RESEARCH USE ONLY
The Carboxylated Surface is designed to facilitate the covalent immobilization of compounds containing reactive free amino groups through EDC-mediated amination. This immobilization method addresses limitations associated with physical adsorption, particularly for small molecules such as peptides (1,000–5,000 Da), drugs, toxins, and hormones. By promoting oriented immobilization, it ensures that the molecules retain both their integrity and accessibility of specific active sites, making these plates ideal for sensitive assays requiring precise molecular interactions.