Goat anti Mouse IgG Plates

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Material

Polystyrene

Format

8 Well Breakable Strip, 8 Well Strip, Solid

Well Volume

Breakable strip: 350 µl Solid: 400 µl Strip: 360 µl

Bottom

Flat

Color

Black, Clear, White

Surface

Goat anti Mouse IgG

Active surface

100 µl

Binding capacity

~ 0.63 pmol/well of Mouse IgG (100 µl)

Sensitivity

~ 12 ng/ml of Mouse IgG (100 µl)

Blocking

Non proteic, Standard

Uniformity

CV < 5 %

Storage

2 – 8 °C

Shelf life

18 months

Packaging

Qty/bag: 1

Trial package

Yes

Minimum order

10 pcs

Unit

5 pcs

Plate Type

Breakable, Solid, Strip

Compatibility

Compatible with most common automation systems

Other information

Certificate of Quality is realeased for every lot

Dimensions

SBS standard

Description

Surface

SURFACE DETAILS

Goat Anti-Mouse IgG Plates are specifically designed to bind the Fc region of mouse immunoglobulin subclasses (IgG1, IgG2a, IgG2b, IgG3) with minimal cross-reactivity to human, bovine, and rabbit serum proteins. These plates are particularly useful for binding assays where antibody quantities are limited, or where antibodies may denature and lose activity when directly adsorbed to polystyrene surfaces.

The Goat Anti-Mouse IgG Coated Plates prevent antibody denaturation caused by direct adsorption on polystyrene, ensuring antibodies remain active. Unlike Protein A or G plates, they provide selective binding specifically to target mouse IgG, offering higher specificity. Additionally, these plates deliver enhanced antibody-binding capacity, especially when using diluted mouse antibody solutions, outperforming direct adsorption to polystyrene.

Secondary Antibodies Goat Anti Mouse IgG Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Goat Anti-Mouse IgG Plates are available in clear, white, and black polystyrene in Breakable Strip format. Strip and Solid format available upon request. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key features of Goat Anti-Mouse IgG Plates

  • Enhanced Binding Capacity: Offers superior antibody-binding capacity compared to direct adsorption on polystyrene, particularly when using diluted mouse antibody solutions.
  • Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
  • Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
  • Wells design: flat bottom.
  • Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
  • Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
    • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
    • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
  • Alphanumeric Coding: For easy and accurate well identification during experiments.
  • Recommended working volume: of 75 to 200 μl
  • Packaging: Each coated plate is packed in a single barrier bag with desiccant
  • Unit: Contains 5 plates
  • Minimum order: 10 plates.
  • Ready to use

Key Benefits

  • Plates Design
    • The mould design provides optical quality, important to reduce the background signal.
    • The rim protects the external face of the bottom from scratches
  • Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
  • Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
  • Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
  • Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Quality and Compliance

  • ISO 9001 Certified: Produced under strict quality protocols.
  • Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
  • SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.

Format Options

Biomat Goat Anti-Mouse IgG Plates are offered in three formats to accommodate diverse laboratory needs:

  1. Goat Anti-Mouse IgG Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required. 
  2. Goat Anti-Mouse IgG Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays. 
  3. Goat Anti-Mouse IgG Plates 96-Well Solid Plates: Solid format designed for high-performance assays. 

Applications

  • Sandwich ELISA: Provides a stable solid support for capturing mouse IgG and detecting non-mouse antibodies.
  • Competitive ELISA: Suitable for quantitative detection of target analytes.
  • IgG Isotyping: Facilitates accurate classification of IgG subclasses.
  • Hybridoma Screening & Selection: Supports efficient screening for monoclonal antibody production.
  • Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
  • Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
  • Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
  • Others

For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.

FOR RESEARCH USE ONLY

Goat Anti-Mouse IgG surface is specifically designed to bind the Fc region of mouse immunoglobulin subclasses (IgG1, IgG2a, IgG2b, IgG3) with high specificity. These plates offer minimal cross-reactivity with human, bovine, and rabbit serum proteins, making them an ideal choice for sandwich ELISA assays that uses goat anti-mouse IgG as the capture antibody and  goat anti-mouse IgG (H+L)-HRP as conjugate.

 

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