Poly-L-lysine Plates utilize their high density of cationic groups to form strong electrostatic and stereospecific bonds with negatively charged molecules. The surface is created using a synthetic molecule rich in α-amino, α-carboxyl, and ε-amino groups, while preserving the polystyrene’s optical properties, making it a versatile tool for a wide range of biological assays. The Poly-L-lysine Coated Plates plates are particularly well-suited for cell adhesion, as they provide an ideal environment for cells to attach due to their positive charge, which interacts with the negatively charged cell membrane. Key applications include interactions with plasminogen and plasminogen activators, ribosomal RNA (rRNA), and double-stranded DNA (dsDNA), making them useful for cell culture, molecular biology, and biochemical studies.
Poly-L-lysine Platesare pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Poly-L-lysine Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.
- Poly-L-lysine surface: It is particularly well-suited for cell adhesion, as it provides an ideal environment for cells to attach due to their positive charge, which interacts with the negatively charged cell membrane.
- It interacts with plasminogen and plasminogen activator
- It interacts with ribosomal RNA
- It interacts with double stranded DNA
- Blocking: Plates are pre blocked with non proteic blocking buffer (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
- Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 75 to 200 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
- Plates Design:
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
- ISO 9001 Certified: Produced under strict quality protocols.
- Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
- SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.
Biomat Poly-L-lysine Plates are offered in three formats to accommodate diverse laboratory needs:
- Poly-L-lysine Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.
- Poly-L-lysine Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.
- Poly-L-lysine Plates 96-Well Solid Plates: Solid format designed for high-performance assays.
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Other
For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.
FOR RESEARCH USE ONLY
The Poly-L-lysine Coated Surface is a versatile tool for a variety of biochemical and molecular biology applications. The positively charged poly-L-lysine surface facilitates strong electrostatic interactions, making it ideal for binding negatively charged molecules. Key applications include interactions with plasminogen and plasminogen activators, as well as binding with ribosomal RNA (rRNA) and double-stranded DNA (dsDNA). This surface is particularly valuable in assays requiring stable attachment of nucleic acids or proteins, providing a reliable platform for studies in gene expression, protein synthesis, and enzyme activity. Its ability to maintain stable molecular interactions makes it a preferred choice for research in cell biology and molecular diagnostics.