Protein A Plates

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115,00160,00 excl. VAT

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Trial package is a smaller package with 5 plates so you can test our products before buying higher quantities

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Material

Polystyrene

Format

8 Well Breakable Strip, 8 Well Strip, Solid

Well Volume

Breakable strip: 350 µl Solid: 400 µl Strip: 360 µl

Bottom

Flat

Color

Black, Clear, White

Surface

Protein A

Active surface

200 µl

Binding capacity

2.66 – 3.33 pmol IgG / well (100 μl), 400 – 500 ng IgG / well (100 μl)

Sensitivity

0.113 ng IgG / well (100 μl)

Blocking

Non proteic, Standard

Uniformity

CV < 10%

Storage

2 – 8 °C

Shelf life

2 years

Packaging

Qty/bag: 1

Trial package

Yes

Unit

5 pcs

Minimum order

10 pcs

Bottom

Flat

Plate Type

Breakable, Solid, Strip

Compatibility

Compatible with most common automation systems

Other information

Certificate of Quality is realeased for every lot

Dimensions

SBS standard

Description

Surface

SURFACE DETAILS

Protein A Plates are specifically designed to capture IgG, either directly or as part of antibody/antigen complexes. The Protein A surface offers an alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA and other plate-based assays. Protein A binds specifically to the Fc region of immunoglobulins from various mammalian species, leaving the F(ab)2 binding sites free for efficient interaction with epitopes.

The Protein A Coated 96-Well Plates enable specific and sterically oriented binding of IgG, making them ideal for a variety of applications. These include the separation of IgG from other immunoglobulins or contaminants, isolation of antigen-antibody complexes, and the analysis and isolation of fusion proteins. Additionally, the plates can be used for the detection and identification of red cell antibodies, particularly when using U-bottom plates, providing a versatile tool for advanced immunoassays and protein analysis.

Protein A Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Protein A Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key features of Protein A Plates

  • Protein A surface:  Captures IgG, either directly or as part of antibody/antigen complexes.
  •  Specific and sterically oriented binding of IgG
  • Separation of IgG from other immunoglobulins or contaminants
  • Isolation of antigen-antibody complexes
  • Analysis and isolation of fusion proteins
  • Detection and identification of red cell antibodies (applicable on U-bottom plates)
  • Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
  • Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
  • Wells design: flat bottom.
  • Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
  • Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
    • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
    • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
  • Alphanumeric Coding: For easy and accurate well identification during experiments.
  • Recommended working volume: of 50 to 100 μl
  • Packaging: Each coated plate is packed in a single barrier bag with desiccant
  • Unit: Contains 5 plates
  • Minimum order: 10 plates.
  • Ready to use

Key Benefits

  • Plates Design
    • The mould design provides optical quality, important to reduce the background signal.
    • The rim protects the external face of the bottom from scratches
  • Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
  • Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
  • Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
  • Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Quality and Compliance

  • ISO 9001 Certified: Produced under strict quality protocols.
  • Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
  • SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.

Format Options

Biomat Protein A Plates are offered in three formats to accommodate diverse laboratory needs:

  1. Protein A Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required. 
  2. Protein A Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays. 
  3. Protein A Plates 96-Well Solid Plates: Solid format designed for high-performance assays. 

Applications

  • Specific and sterically oriented binding of IgG
  • Separation of IgG from other immunoglobulins or contaminants
  • Isolation of antigen-antibody complexes
  • Analysis and isolation of fusion proteins
  • ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
  • Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
  • Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
  • Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
  • Other

For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.

FOR RESEARCH USE ONLY

The Protein A Coated Surface on Protein A Coated 96 Well Solid Plates is specifically designed to capture IgG, either directly or as part of antigen/antibody complexes. These plates are ideal for applications such as the specific and sterically oriented binding of IgG, the separation of IgG from other immunoglobulins or contaminants, the isolation of antigen-antibody complexes, and the analysis of fusion proteins. This versatile platform ensures efficient and reliable results in immunoassays requiring precise IgG binding and separation.

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