Protein A/G Coated Plates provide an efficient alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA assays. The surface of these plates is designed to bind a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD. Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, offering versatility in binding different immunoglobulins. Unlike Protein A alone, Protein A/G has reduced pH dependency, while combining the beneficial properties of both Protein A and Protein G, making it a powerful tool for various immunoassays.
Protein A/G Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.
Protein A/G Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.
- Protein A/G surface: It binds a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD
- specific and sterically oriented bond of antibodies
- purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
- highest specificity and capacity
- retains antibody activity and orients antibody for maximum binding
- generally not suitable for sandwich ELISA assays
- Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
- Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
- Wells design: flat bottom.
- Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
- Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
- Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
- Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
- Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
- Alphanumeric Coding: For easy and accurate well identification during experiments.
- Recommended working volume: of 50 to 100 μl
- Packaging: Each coated plate is packed in a single barrier bag with desiccant
- Unit: Contains 5 plates
- Minimum order: 10 plates.
- Ready to use
- Plates Design:
- The mould design provides optical quality, important to reduce the background signal.
- The rim protects the external face of the bottom from scratches
- Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
- Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
- Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
- Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.
- ISO 9001 Certified: Produced under strict quality protocols.
- Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
- SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.
Biomat Protein A/G Plates are offered in three formats to accommodate diverse laboratory needs:
- Protein A/G Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.
- Protein A/G Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.
- Protein A/G Plates 96-Well Solid Plates: Solid format designed for high-performance assays.
- Specific and sterically oriented bond of antibodies
- Purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
- ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
- Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
- Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
- Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
- Other
For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.
FOR RESEARCH USE ONLY
The Protein A/G Coated Surface on Protein A/G Coated 96 Well Solid Plates is designed to bind a wide range of IgG species, including all subclasses of mouse IgG (except IgA, IgM, or serum albumin) and all subclasses of human IgG, as well as IgA, IgE, IgM, and IgD. This versatile product is ideal for the purification and detection of mouse monoclonal IgG antibodies, as well as the purification of macaque IgG, offering a powerful tool for immunoassays and antibody-based applications.