Streptavidin Plates

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Material

Polystyrene

Format

8 Well Breakable Strip, 8 Well Strip, Solid

Well Volume

Breakable strip: 350 µl Solid: 400 µl Strip: 360 µl

Bottom

Flat

Color

Black, Clear, White

Surface

Streptavidin

Active surface

200 µl

Binding capacity

Biotin = ~ 12 – 14 pmol/well (200 μl), IgG-b = 100 ng/ well (100 μl)

Blocking

Non proteic, Standard

Uniformity

CV < 5 %

Storage

2 – 8 °C

Shelf life

3 years

Packaging

Qty/bag: 1

Minimum order

10 pcs

Trial package

Yes

Unit

5 pcs

Plate Type

Breakable, Solid, Strip

Dimensions

SBS standard

Other information

Certificate of Quality is realeased for every lot

Compatibility

Compatible with most common automation systems

Description

Surface

SURFACE DETAILS

The Streptavidin Plates are ideal for binding a wide range of biotinylated molecules, making them highly recommended for ELISA and other immunoassays. Streptavidin is one of the most popular biotin-binding proteins in research, with each of its four subunits capable of binding a biotin molecule, resulting in enhanced assay sensitivity and reliability. The streptavidin-coated surface provides a versatile and powerful platform for capturing biotinylated antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, DNA fragments, and more. This coating is especially useful for molecules that may not adhere reliably through passive adsorption or that require specific orientation for optimal binding. Using Streptavidin Coated Plates for ELISA ensures improved assay performance, with higher sensitivity and reduced background noise, leading to more accurate and consistent results.

Streptavidin Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Streptavidin Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key features of Streptavidin Plates

  • Streptavidin-coated surface: Versatile platform for capturing a wide range of biotinylated molecules, including antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments.Ideal for binding molecules that: 
    • do not adhere reliably through passive adsorption
    • require specific orientation for optimal binding.
  • Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
  • Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
  • Wells design: flat bottom.
  • Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
  • Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
    • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
    • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
  • Alphanumeric Coding: For easy and accurate well identification during experiments.
  • Recommended working volume: of 75 to 200 μl
  • Packaging: Each coated plate is packed in a single barrier bag with desiccant
  • Unit: Contains 5 plates
  • Minimum order: 10 plates.
  • Ready to use

Key Benefits

  • Plates Design
    • The mould design provides optical quality, important to reduce the background signal.
    • The rim protects the external face of the bottom from scratches
  • Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
  • Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
  • Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
  • Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Quality and Compliance

  • ISO 9001 Certified: Produced under strict quality protocols.
  • Certificate of Quality: All lots are tested for uniformity and reproducibility and a certificate is released for every lot.
  • SBS Standards: Designed for compatibility with automated processing systems, ensuring ease of use in high-throughput labs.

Format Options

Format Options

Biomat Streptavidin Plates are offered in three formats to accommodate diverse laboratory needs:

  1. Streptavidin Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required. 
  2. Streptavidin Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.
  3. Streptavidin Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Applications

  • Specific and sterically oriented bond of antibodies
  • Purification and detection of mouse monoclonal IgG antibodies and for purification of macaque IgG
  • Highest specificity and capacity
  • Retains antibody activity and orients antibody for maximum binding
  • ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
  • Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
  • Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
  • Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.
  • Other

For further information on the various Biomat 96-Well Plate formats and their specific use cases, contact us.

FOR RESEARCH USE ONLY

The Streptavidin Coated Surface on Streptavidin Coated 96 Well Solid Plates provides a highly effective and versatile platform for binding biotinylated molecules, including proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. Streptavidin’s strong affinity for biotin allows for robust and stable interactions, making it particularly valuable for applications where passive adsorption is unreliable or where molecules may adsorb in an unfavorable orientation. These plates ensure consistent and oriented binding, which is critical for enhancing assay sensitivity and accuracy in ELISA, nucleic acid capture, and other biochemical assays. Additionally, the high specificity of streptavidin-biotin binding minimizes background noise, offering improved signal clarity in complex assay systems.

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