Anti Human Immunoglobulins

Anti human IgG/IgM/IgA coated

Antibodies anti human immunoglobulins Coated Surface, respectively with Goat Anti human IgG , Goat Anti human IgM and Goat Anti human IgA , are ideal for the determination of the respective immunoglobulins in human biological liquids ( serum, plasma, urine, cerebrospinal fluid, saliva and others ).

Features of Anti human IgG/IgM/IgA Coated 96 Well Plate:

  • typically used for ELISA sandwich assays
  • extremely specific since they bind the respective immunoglobulins via the Fc fragment : γ for IgG, µ for IgM and α for IgA
  • truly reliable to determine the minimum quantity ( sensitivity ) of the respective immunoglobulins
  • have a high binding affinity for the respective immunoglobulins

Biomat’s Anti human IgG/IgM/IgA Coated 96-Well Plates product

These are the available configurations of Anti human IgG/IgM/IgA  96-Well Coated Plates products by Biomat.

Click on the configuration link to scroll down the page to the relative section. (COLLEGARE ALLE RISPETTIVE PAGINE DEI PRODOTTI)

Anti human IgG/IgM/IgA Coated Surface main features

Biomat Coated Plates with Anti human IgG/IgM/IgA  are suitable with ELISA Assay Technology due to these features:

  • Ready to use
  • Manufactured under ISO:9001 guidelines
  • Made in pure polystyrene with low fluorescence
  • Post-coated (blocked) for low non specific binding and long-term stability
  • Alphanumeric coding for easy well recognition
  • Microplates comply with SBS standards and the design assures a good performance in automatic processing plant
  • Certificate of Quality is released for every lot
  • for Research Use Only

Also, Anti human IgG/IgM/IgA Coated 96-Well Plates ensure:

Uniformity

The Anti human IgG/IgM/IgA Coated products show a CV% less than 10 when used as a catcher of the respective immunoglobulins in a sandwich ELISA format using the anti human counterpart ( H + L )-HRP as detector and TMB as substrate.

Storage and Stability

The microplates, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Anti human IgG/IgM/IgA Coated Surfaces Product specifications

Goat Anti Human IgG Fcγ

An affinity purified goat anti human IgG specifically binds the Fc region of human IgG immunoglobulins, with minimal cross-reaction to human IgM and IgA, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgG ( H+L )-HRP detection antibody.

Goat Anti Human IgG Fcγ Coated 96-Well Plates Product Specifications

Coating

Affinity purified goat anti human IgG Fcγ is coated using 100 μl/well.

Binding capacity

Microplate was saturated with human IgG at a concentration of 0.500 μg/ml ( 50 ng/well) in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.

Results:50 ng/well of human IgG

Sensitivity

Human IgG was detected at a concentration significantly above background in an ELISA format using goat anti human IgG (H+L)-HRP as detector and TMB as substrate.

Results:0.1 ng/well of human IgG

see Binding capacity and Sensitivity technical note Anti Human IgG (NT52)

Goat Anti Human IgM Fcµ

An affinity purified goat anti human IgM specifically binds the Fc region of human IgM immunoglobulins , with minimal cross reaction to human IgG and IgA, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgM ( H+L ) detection antibody.

Goat Anti Human IgM Fcµ Coated 96-Well Plates Product Specifications

Coating

Affinity purified Goat anti human IgM Fcµ is coated using 100 μl/well.

Binding capacity and Sensitivity

Microplate was saturated with human IgM at a concentration of  about 3.0 μg/ml (300 ng/well) in an ELISA format using goat anti human IgM (H+L)-HRP as detector and TMB as substrate.

Results:≤ 300 ng/well of human IgM

Sensitivity

Human IgM was detected at a concentration significantly above background in an ELISA format using goat anti human IgM ( H+L )-HRP as detector and TMB as substrate.

Results:10 ng/ml of human IgM

see Binding capacity and Sensitivity technical note Anti Human IgM (NT54)

Goat Anti Human IgA Fcα

An affinity purified goat anti human IgA specifically binds the Fc region of human IgA immunoglobulins , with minimal cross reaction to human IgG and IgM, mouse, bovine and rabbit serum proteins.

These plates may be used as solid support for sandwich ELISA assay utilizing a goat anti human IgA ( H+L ) detection antibody.

Goat Anti Human IgA Fcα Coated 96-Well Plates Product Specifications

Coating

Affinity purified Goat anti human IgA Fcα is coated using 100 μl/well.

Binding capacity

Microplate was saturated with human IgA at a concentration of about 1.0 μg/ml (100 ng/well) in an ELISA format using goat anti human IgA (H+L)-HRP as detector and TMB as substrate.

Results:≤ 100 ng/well of human IgA

Sensitivity

Human IgA was detected at a concentration significantly above background in an ELISA format using goat anti human IgA ( H+L )-HRP as detector and TMB as substrate.

Results:0.700 ng/ml of human IgA

see Binding capacity and Sensitivity technical note Anti Human IgA (NT53)

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