Protein G

Protein G Coated Surface offers a powerful and sensitive instrument designed for the capture of IgG applied directly or as antibody/antigen complex.

Protein G Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.

Protein G (Recombinant Protein G – M.W. 26.1 kDa) from Streptococcus sp., expressed in E. coli, specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) , with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated onto microplates, the Protein G can securely capture IgG applied directly or as antigen/antibody complexes.

Examples of applications for Protein G Coated surface

  • specific and sterically oriented bond of IgG
  • separation of IgG from other immunoglobulins
  • separation of antigen-antibodies complexes
  • isolation and analysis of fusion proteins

Biomat Protein G Coated Products

96-Well Plates

Other 96-Well Plates

PCR Products

Other PCR Products

Protein G Coated Surfaces main features

Biomat Protein G Coated products have the following characteristics:

  • Ready to Use
  • Manufactured under ISO:9001 guidelines
  • All lots are tested for uniformity and reproducibility
  • Certificate of Quality is released for every lot
  • For Research Use Only

Also, Protein G  Coated Products ensures:

Uniformity

The Protein G Coated Products  show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability

The Protein G Coated products, if unopened,  are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

Protein G Coated Microplates Specifications

Coating

Recombinant Protein G ( M.W. 26.1 kDa ), from Streptococcus sp., expressed in E. coli, is coated using 200 µl/well.

Binding capacity

Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

see Binding Capacity technical note

Results:2.66 – 3.33 pMol IgG/well (100 μl volume)

Sensitivity

Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

see Sensitivity technical note

Results:0.056 ng/well of human IgG

Protein G Coated PCR 8 Strip Tubes and PCR Plates Specifications

Coating

Recombinant Protein G ( M.W. 26.1 kDa ), from Streptococcus sp., expressed in E. coli, is coated using 100 µl/tube.

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