Maleimide Coated Surface offers a powerful instrument for binding biomolecules containing free sulfhydryl groups or reducible disulfide bonds that are difficult to coat onto polystyrene plates.
Maleimide Coated Surfaces offer a powerful instrument for binding biomolecules containing free sulfhydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens ), or reducible disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.
At pH 6.5-7.5 maleimide reacts with free sulfhydryl groups to yield stable bonds, while the reaction with amine becomes significant at pH > 7.5.
If sulfhydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfhydryls for allowing interaction with maleimide.
Biomat Maleimide Coated Products
96-Well Plates
Other 96-Well Plates
PCR Products
Other PCR Products
Maleimide Coated Surfaces main features
Biomat Maleimide Coated products have the following characteristics:
- Ready to Use
- Manufactured under ISO:9001 guidelines
- All lots are tested for uniformity and reproducibility
- Certificate of Quality is released for every lot
- For Research Use Only
Also, Maleimide Coated Products ensures:
Uniformity
The Maleimide Coated Products show a CV% less than 10 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate
Storage and Stability
The Maleimide Coated products, if unopened, are stable at 2 – 8 °C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.
Maleimide Coated Microplates Specifications
Coating
A derived maleimide is coated using 100 μl/well.
Binding capacity
Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.
see Binding Capacity technical note
Maleimide Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
A derived maleimide is coated using 100 μl/tube.